HTPQA

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HTPQA熒光探針 優點：
HTPQA熒光探針 是一種酶熒光成像探針，實現了活細胞內堿性磷酸酶活性的原位成像檢測。該合成的堿性磷酸酶探針具有靈敏度高、選擇性好等優點。該探針能夠解決傳統水溶性探針信號易擴散、衰減的問題，從而實現堿性磷酸酶的原位成像檢測，除此之外，還成功應用于成像檢測骨肉瘤細胞及組織中堿性磷酸酶的活性。這一新型固態發光成像探針的開發呈現出固態發光熒光染料在蛋白酶原位成像檢測中的巨大優勢。

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Enzyme-responsive, fluorogenic probes fail to provide in situ information because the released fluorophores tend to diffuse away from the reaction sites. The problem of diffusive signal dilution can be addressed by designing a probe that upon enzyme conversion releases a fluorophore that precipitates. An excited-state intramolecular proton transfer (ESIPT)-based solid-state fluorophore HTPQ was developed that is strictly insoluble in water and emits intense fluorescence in the solid state, with λex/em=410/550 nm, thus making it far better suited to use with a commercial confocal microscope. HTPQ was further utilized in the design of an enzyme-responsive, fluorogenic probe (HTPQA), targeting alkaline phosphatase (ALP) as a model enzyme. HTPQA makes possible diffusion-resistant in situ detection of endogenous ALP in live cells. It was also employed in the visualizing of different levels of ALP in osteosarcoma cells and tissue, thus demonstrating its interest for the diagnosis of this type of cancer.

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       目標探針HTPQA（紅色信號通道）與商品化ALP探針4-MUP在HeLa細胞中共同孵育的成像圖。從成像結果可以看出：（A, B）HTPQA能夠實現ALP的原位檢測，而4-MUP則擴散至整個細胞質；（C, D）HTPQA信號穩定，長時間監測信號無明顯擴散，而4-MUP的信號逐漸消失。

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