鈉離子指示劑介紹
鈉離子指示劑允許在存在生理濃度的其他單價(jià)陽離子的情況下對 Na+ 濃度進(jìn)行空間和時(shí)間分辨率。 這些鈉指示劑在結(jié)合 Na+ 時(shí)表現(xiàn)出綠色熒光發(fā)射強(qiáng)度的增加，而波長幾乎沒有變化。

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鈉離子指示劑
※ 測量 Na+ 濃度的穩(wěn)態(tài)變化
※ 跟蹤軸突和突觸前末端的 Na+
指示劑可用于估計(jì)離體線粒體中的 Na+ 梯度、測量細(xì)胞內(nèi) Na+ 水平、測量細(xì)胞內(nèi) Na+ 流出，以及與其他熒光指示劑結(jié)合，將細(xì)胞內(nèi) Na+ 的變化與 Ca2+ 和 Mg2+ 濃度、細(xì)胞內(nèi) pH 值和膜相關(guān)聯(lián)潛在的。

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鈉指示劑選擇指南

CoroNa Green	CoroNa Green, AM		SBFI, Tetraammonium Salt		SBFI, AM
Readout	Increase in green fluorescence emission intensity upon binding Na+, with little shift in wavelength		Spectral excitation shift used to estimate Na+聽gradients in isolated mitochondria and intracellular Na+聽levels
Range	Responds to a range of intracellular Na+around 10 mM		Responds to Na+in physiological range
Fluorophore	CoroNa Green		SBFI
Standard filter set	FITC		Requires excitation ratio to quantify Na+levels
Ex/Em (nm)	~492/516		~340 and 380/500
Bibliography	Citations		Citations
Cell permeant or impermeant?	Impermeant	Cell permeant	Impermeant	Cell permeant
Platforms	Imaging	Imaging	Imaging	Imaging
Usage notes	Load by microinjection, patch pipette, or pinocytic loading agent	Load in cell media	Load by microinjection, patch pipette, or pinocytic loading agent	Load in cell media
Format	1 mg	50ug	1 mg	1 mg	50ug
Cat. No.

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